Yes, you can limit file upload size, although there is already a 150 MB limit cap. This means that form users cannot upload files bigger than 150 MB each. You can opt for an upload field which accepts multiple files.
To limit file upload size, go to your Form Editor, click on the File Upload field in your form and its Edit Field panel will open up on the left side of the page. In the Maximum Upload Size box, type your preferred maximum size (in KB). If you want to add a minimum upload size limit, type in the Minimum Upload Size box. That’s it.